Regulatory

Part:BBa_J119442:Design

Designed by: Todd Eckdahl   Group: Eckdahl Lab   (2019-07-18)


RET Promoter i-Motif and G-quadruplex


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

I had to design 5' sticky ends that would allow the part to be ligated into a plasmid with either the G-quadruplex or the i-motif on the coding strand.


Source

This part comes from the human genome. It is located in the promoter of the human RET oncogene.

References